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KMID : 0379119920200030234
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Korean Journal of Mycology
1992 Volume.20 No. 3 p.234 ~ p.239
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Molecular Analysis of double - stranded RNA in Abnormal Growing Oyster - Mushrooms , Pleurotus florida and P. ostreatus due to Virus Infection
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박용환/Park, Yong Hwan
신관철/고승주/Wessels, J. G. H./Shin, Gwan Chull/Go, Seung Joo
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Abstract
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The experiment was performed to find out the possibilities to detect virus infection in oyster mushrooms, Pleurotus species by analysis of doublestranded ribonucleic acid (ds RNA). Ds RNA segments were extracted from virus infected isolates which grew abnormally. But virus free isolates didn´t show any ds RNA segments. The ds RNA was consisted of one Large segment of 8100 base pairs (bp) and 4 smaller segments with 2170, 2120, 1980 and 1984 bp. Whereas, cell free virus particles showed only one larger ds RNA segment. The ds RNA was dissolved by RNase A in low salt, 0.1 M SSC and melted at 85℃. It was possible to use the ds RNA analysis for detecting virus infection directly from the host cells.
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